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KMID : 1024420070110040279
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Food Engineering Progress
2007 Volume.11 No. 4 p.279 ~ p.283
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Growth Activity of Bifidobacterium spp. by D.Ps of Locust Bean Gum Galactomannan Hydrolysatesfrom Trichoderma harzianum β-mannanase
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Park Seong-Eun
Lee Hee-Jung
Kim Soon-Ok
Gang Jong-Back
Park Gwi-Gun
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Abstract
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Locust bean gum galactomannan was hydrolyzed to degree of polymerization (D.P.) 2, 4, 7 galactosyl mannooligosaccharides by the purified β-mannanase. To investigate the effects of locust bean gum galactosyl mannooligosaccharides on in vitro growth of Bifidobacterium longum, B. bjfidum, B. infantis, B. adolescentis, B. animalis, and B. breve, Bifidobacterium spp. were cultivated individually on the modified-MRS medium containing carbon source such as D.P 2, 4 and 7 galactosyl mannoolgosaccharides, respectively. B. longum and B. bifidum grew up 9.0-fold and 6.6-fold more effectively by the treatment of D.P 4 galactosyl mannooligosaccharide, compared to those of standard MRS medium. Especially, hetero type of D.P 4 and 7 were more effective than homo type of D.P 2 on the growth of Bifidobacterium spp and Lactobacillus spp.
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KEYWORD
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Locust bean gum galactomannan, β-mannanase, Bifidobacterium spp.
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